Module 1
Introduction to Digital Images
Basic concepts of digital images, file format, color scheme
Fundamentals of Image Processing
Histograms, displays, pixel adjustment, filters, kernels 
Fourier Transformation
Image processing in Fourier space
Module 2
Object-based Colocalization Analysis
​Quantifying overlapping objects
Pixel-based Colocalization and intensity Analysis
Colocalization coefficients, ratiometric imaging 
Analysis of Biological Movement
Fluorescence recovery rate, particle tracking, motion analysis
Object Segmentation
Turning pixel map into discrete objects
Module 3
Accurate and Sufficient Scientific Reporting
The effects of inaccurate and insufficient documentation, and what constitute good scientific reporting
Object Segmentation with Machine Learning
Trainable Weka Segmentation in 2D and 3D

Quantitative Fluorescence Microscopy Workshop 2022

FIJI Image Processing and Analysis Workshop

June 29-30, 2022
FAES, National Institutes of Health

Download Links
FIJI Program
Click here to download for Mac
Click here to download for Windows
Click here to download for Linux

You should use this version even if you already have FIJI installed on your laptop. Do not update FIJI after installation.
Reading List
For additional reading, click here.
Macro Examples
Save this folder onto your desktop.
Sample Images
Save this folder onto your desktop