AGENDA
Module 1
Introduction to Digital Images
Basic concepts of digital images, file format, color scheme
Fundamentals of Image Processing
Histograms, displays, pixel adjustment, filters, kernels 
Fourier Transformation
Image processing in Fourier space
Module 2
Object-based Colocalization Analysis
​Quantifying overlapping objects
Pixel-based Colocalization and intensity Analysis
Colocalization coefficients, ratiometric imaging 
Analysis to track changes and movement
Fluorescence recovery rate, particle tracking, motion analysis
Object Segmentation
Turning pixel map into discrete objects
Module 3
Introduction to Fiji Macros
Basic programming concepts: variables, boolean logic, and user dialogs 
Intermediate Fiji Macros
Automating with loops, code reuse using functions, and summarizing data
Object Segmentation by Machine Learning
Turning pixel map into discrete objects
 
FIJI Program
Click here to download for Mac
Click here to download for Windows
Click here to download for Linux
You should use this version even if you already have FIJI installed on your laptop. Do not update FIJI after installation.
Reading List
For additional reading, click here.
Macro Examples
Save this folder onto your desktop.
Sample Images
Save this folder onto your desktop
 
 
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